Flow cytometric analysis of Mouse CD95 expression on C57BL/6 mouse thymocytes. C57BL/6 mouse thymocytes were stained with either Alexa Fluor® 647 Armenian hamster IgG Isotype Control (Left panel) or SDT Alexa Fluor® 647 Armenian hamster Anti-Mouse CD95 Antibody (Right panel) at 1.25 μl/test. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Alexa Fluor® 647 Armenian hamster Anti-Mouse CD95 Antibody (S-R535)
Alexa Fluor® 647 Armenian hamster Anti-Mouse CD95 Antibody (S-R535)
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Product Details
Product Details
Product Specification
| Host | Armenian hamster |
| Antigen | CD95 |
| Synonyms | Tumor necrosis factor receptor superfamily member 6; Apo-1 antigen; Apoptosis-mediating surface antigen FAS; FASLG receptor; Apt1; Tnfrsf6; Fas |
| Location | Cell membrane |
| Accession | P25446 |
| Clone Number | S-R535 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse thymocytes |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume | Ms |
Background
CD95, also known as Fas or APO-1, is a 48 kDa type-I transmembrane receptor of the TNF-receptor superfamily that, upon trimerization by its cognate ligand CD95L (FasL), assembles the death-inducing signaling complex (DISC) by recruiting the adaptor FADD and procaspase-8/10, triggering a caspase cascade that culminates in apoptosis, thereby governing immune homeostasis, peripheral tolerance, and elimination of infected or oncogenic cells, while dysregulation of this pathway contributes to autoimmune lymphoproliferative syndrome, cancer immune evasion, and resistance to chemotherapy.
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