WB result of SIRT6 Rabbit pAb
Primary antibody: SIRT6 Rabbit pAb at 1/500 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: HCT 116 whole cell lysate 20 µg
Lane 4: K562 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 40 kDa
SIRT6 Rabbit Polyclonal Antibody
SIRT6 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SIRT6 |
| Synonyms | NAD-dependent protein deacylase sirtuin-6; NAD-dependent protein deacetylase sirtuin-6; Protein mono-ADP-ribosyltransferase sirtuin-6; Regulatory protein SIR2 homolog 6 1 publication (hSIRT6); SIR2-like protein 6; SIR2L6 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | Q8N6T7 |
| Isotype | IgG |
| Application | WB, ICC, ICFCM, IP |
| Reactivity | Hu |
| Predicted Reactivity | Mq |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500 | |
| IP | 1:50 | |
| ICC | 1:500 | |
| ICFCM | 1:500 |
Background
SIRT6 is a multifaceted protein with diverse roles in cellular processes, including aging, metabolism, inflammation, and cardiovascular diseases. As a NAD+-dependent enzyme, it possesses deacetylase, ADP-ribosyltransferase, and long-chain fatty acid deacylase activities, which enable it to regulate various biological functions. In the context of aging, SIRT6 has been recognized for its role in genomic stability, telomere maintenance, and DNA repair, earning it a reputation as a longevity-associated protein. It contributes to the preservation of telomere integrity by deacetylating histone H3 at lysine 9 (H3K9), which helps stabilize the chromatin structure near telomeres. Metabolically, SIRT6 plays a crucial role in glucose and lipid homeostasis. SIRT6 also has a significant impact on inflammation and oxidative stress. It can reduce inflammation by inhibiting the NF-κB pathway and promote antioxidant responses through the NRF2-HO-1 pathway, thereby protecting cells from oxidative damage. Conversely, SIRT6 has also been shown to promote the secretion of pro-inflammatory cytokines like TNF-α, indicating a complex role in inflammatory responses. In cardiovascular health, SIRT6 is protective against diseases such as atherosclerosis, cardiac hypertrophy, and heart failure. It can prevent endothelial dysfunction, reduce vascular inflammation, and promote cardiac cell survival under stress conditions. The protective effects of SIRT6 in the cardiovascular system are attributed to its ability to modulate gene expression and metabolic pathways that are critical for maintaining cardiac function and vascular integrity.
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Western Blot
FC
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling SIRT6 antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
IP
SIRT6 Rabbit pAb at 1/50 dilution (1 µg) immunoprecipitating SIRT6 in 0.4 mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using SIRT6 Rabbit pAb at 1/500 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 40 µg (Input)
Lane 2: SIRT6 Rabbit pAb IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
Predicted MW: 39 kDa
Observed MW: 42 kDa
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- SIRT6 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
