Standard curve
Example of Human CEA standard curve in Assay Diluent #12.
Human CEA OneStep ELISA Kit
Human CEA OneStep ELISA Kit
Price:
Regular price
$535.00 USD
Regular price
Sale price
$535.00 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Antigen | CEA |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Hu |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 1.4%； Inter-assay: 2.3% |
| Sample type | Cell culture supernatant |
| Assay type | Sandwich (quantitative), Serum, Plasma |
| Sensitivity | 1.004 pg/mL |
| Range | 0.156 ng/mL – 10 ng/mL |
| Recovery | Cell culture supernatant: 97% Serum: 102% Plasma: 103% |
| Assay time | 60 minutes |
| Species reactivity | Hu |
Background
CEA (carcinoembryonic antigen) is an acidic glycoprotein with the characteristics of human embryo antigen. It exists on the surface of cancer cells derived from endodermal cells and is a structural protein of cell membrane. This protein is formed in the cytoplasm and secreted into the surrounding body fluids. Therefore, it can be detected in various body fluids and excreta such as serum, cerebrospinal fluid, breast milk, gastric juice, pleural and abdominal fluid, urine and feces. As a specific marker for the early diagnosis of colon and rectal cancer, CEA level is found to increase not only in gastrointestinal malignancies, but also in serum of breast cancer, lung cancer and other malignancies through a large number of clinical practices.Therefore, CEA is a broad-spectrum tumor marker. Although it cannot be used as a specific index for the diagnosis of certain malignant tumors, it still has important clinical value in the differential diagnosis, disease monitoring and efficacy evaluation of malignant tumors.This product is the recombinant human CEA protein expressed from human 293 cells (HEK293).
Picture
Picture
ELISA
Linearity
The concentrations of Human CEA were measured and interpolated from the target standard curves and corrected for sample dilution.
The sample is undiluted samples are as follows: MCF-7 cells Extract was 20fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 10mg/mL in MCF-7 cells Extract.
Linearity
The concentrations of Human CEA were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: Human serum was 2fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 1184.44 pg/mL in Human serum.
#2 sample is undiluted samples are as follows: Human serum was 2fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 945.65 pg/mL in Human serum.
To assess the linearity of the assay, two samples were spiked with high concentrations of Human CEA in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
To assess the linearity of the assay, the sample was spiked with high concentrations of Human CEA in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
