Standard curve
Example of Human IL-6 standard curve in Assay Diluent #1.
Human IL-6 Surpass ELISA PairSet Kit
Human IL-6 Surpass ELISA PairSet Kit
Price:
Regular price
$970.00 USD
Regular price
Sale price
$970.00 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Antigen | IL-6 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Human |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 3.2%； Inter-assay: 3.8% |
| Sample type | Cell culture supernatant, Serum，Plasma |
| Assay type | Sandwich(quantitative) |
| Sensitivity | 0.144pg/ml |
| Range | 0.94pg/mL – 60pg/mL |
| Recovery | Cell culture supernatant: 96% Serum: 83% Plasma: 83% |
| Assay time | 2.5 hours |
| Species reactivity | Human |
Background
Interleukin 6 (IL-6) is a prototypical cytokine for maintaining homeostasis. When homeostasis is disrupted by infections or tissue injuries, IL-6 is produced immediately and contributes to host defense against such emergent stress through activation of acute-phase and immune responses. However, dysregulated excessive and persistent synthesis of IL-6 has a pathological effect on, respectively, acute systemic inflammatory response syndrome and chronic immune-mediated diseases.
Picture
Picture
ELISA
Linearity
The concentrations of IL-6 were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: human PBMC cells stimulated with 10ug/ml PHA for 5days (0.5%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 88,750 pg/mL in stimulated human PBMC supernatant.
#2 sample is undiluted samples are as follows: human PBMC cells culture for 5days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 46 pg/mL in unstimulated human PBMC supernatant.
Leading Competitor comparison
