WB result of Runx3 Rabbit mAb
Primary antibody: Runx3 Rabbit mAb at 1/500 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: SW620 whole cell lysate 20 µg
Negative control: MCF7 whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 48 kDa
(This blot was developed with high sensitivity substrate)
Runx3 Recombinant Rabbit mAb (S-R356)
Runx3 Recombinant Rabbit mAb (S-R356)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Synonyms | Runt-related transcription factor 3, Acute myeloid leukemia 2 protein, Core-binding factor subunit alpha-3 (CBF-alpha-3), Oncogene AML-2, Polyomavirus enhancer-binding protein 2 alpha C subunit (PEA2-alpha C; PEBP2-alpha C), SL3-3 enhancer factor 1 alpha C subunit, SL3/AKV core-binding factor alpha C subunit, AML2, CBFA3, PEBP2A3 |
| Location | Cytoplasm, Nucleus |
| Accession | Q13761 |
| Clone Number | S-R356 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IP |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500 | null |
| IHC-P | 1:500 | null |
| IP | 1:50 | null |
Background
Runx3 is a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-YGYGGT-3' found in a number of enhancers and promoters,[6] and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer.
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Picture
Western Blot
IP
Runx3 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Runx3 in 0.4 mg SW620 whole cell lysate.
Western blot was performed on the immunoprecipitate using Runx3 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: SW620 whole cell lysate 10 µg (Input)
Lane 2: Runx3 Rabbit mAb IP in SW620 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in SW620 whole cell lysate
Predicted MW: 44 kDa
Observed MW: 48 kDa
（This blot was developed with high sensitivity substrate）
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human thymus. Anti-Runx3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Runx3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Runx3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-Runx3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human Hodgkin’s lymphoma. Anti-Runx3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
