WB result of Phospho-p53 (Ser15) Rabbit pAb
Primary antibody: Phospho-p53 (Ser15) Rabbit pAb at 1/2000 dilution
Lane 1: untreated A431 whole cell lysate 20 µg
Lane 2: A431 treated with 1 µg/ml doxorubicin for 24 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 50 kDa
Phospho-p53 (Ser15) Rabbit Polyclonal Antibody
Phospho-p53 (Ser15) Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-p53 (Ser15) |
| Synonyms | Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; P53; TP53 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P04637 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu |
| Purification | Immunogen Affinity |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000-1:2000 | Hu |
| ICC | 1:500 | Hu |
Background
Phospho-p53 (Ser15) is a critical biomarker of p53 activation, playing a key role in cellular responses to DNA damage and stress. When cells experience DNA damage, p53 is phosphorylated at serine 15, which stabilizes the protein and enhances its transcriptional activity. This phosphorylation event triggers a cascade of downstream effects, including cell cycle arrest, DNA repair, and apoptosis, depending on the severity of the damage. In senescent cells, phospho-p53 (Ser15) is often localized in the nucleus, where it interacts with other proteins such as FOXO4 to maintain the senescent state and prevent apoptosis. This regulatory mechanism is crucial for understanding the balance between cellular senescence and apoptosis, and it has significant implications for aging, cancer, and other age-related diseases.
Picture
Picture
Western Blot
Dot Blot
Dot blot result of Phospho-p53 (Ser15) Rabbit pAb
Lane 1: p53 (Ser15) phospho peptide
Lane 2: p53 unmodified peptide
Primary antibody: Phospho-p53 (Ser15) Rabbit pAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Immunocytochemistry
ICC analysis of A431 cells treated with doxorubicin (1 µg/ml, 24h) (top panel) and untreated A431 cells (below panel). Anti- Phospho-p53 (Ser15) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
