WB result of Phospho-Histone H3 (Ser10) Recombinant Rabbit mAb
Primary antibody: Phospho-Histone H3 (Ser10) Recombinant Rabbit mAb at 1/20000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 ng/ml Nocodazole for 18 hours and 100 nM Calyculin A for 1 hour whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
Phospho-Histone H3 (Ser10) Recombinant Rabbit mAb (S-960-58)
Phospho-Histone H3 (Ser10) Recombinant Rabbit mAb (S-960-58)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-Histone H3 (Ser10) |
| Synonyms | H3S10P |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P68431 |
| Clone Number | S-960-58 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ChIP |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Ar, Bv, C.el, Dr, Hm, Or, Xe, Ys |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:10000 | |
| WB | 1:20000 | Hu, Ms, Rt |
| ChIP | 1:20-1:50 | Hu, Ms, Rt |
Background
Phospho-Histone H3 (Ser10) is a critical epigenetic marker associated with chromosome condensation and mitotic progression. This phosphorylation event typically occurs during the G2 to M phase transition and is essential for chromatin condensation, making it a specific indicator of mitotic cells. It is regulated by kinases such as Aurora B and plays a significant role in mitotic chromosome condensation and decondensation. Additionally, H3 Ser10 phosphorylation has been implicated in transcriptional regulation and early gene expression, and its dysregulation has been linked to neoplastic cell transformation and cancer.
Picture
Picture
Western Blot
WB result of Phospho-Histone H3 (Ser10) Recombinant Rabbit mAb
Primary antibody: Phospho-Histone H3 (Ser10) Recombinant Rabbit mAb at 1/20000 dilution
Lane 1: HeLa treated with 100 ng/ml Nocodazole for 18 hours and 100 nM Calyculin A for 1 hour whole cell lysate 20 µg
Lane 2: HeLa treated with 100 ng/ml Nocodazole for 18 hours and 100 nM Calyculin A for 1 hours, then treated with phosphatase whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
WB result of Phospho-Histone H3 (Ser10) Recombinant Rabbit mAb
Primary antibody: Phospho-Histone H3 (Ser10) Recombinant Rabbit mAb at 1/20000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 strave 3 hours, then treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
WB result of Phospho-Histone H3 (Ser10) Recombinant Rabbit mAb
Primary antibody: Phospho-Histone H3 (Ser10) Recombinant Rabbit mAb at 1/20000 dilution
Lane 1: untreated C6 whole cell lysate 20 µg
Lane 2: C6 treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
Dot Blot
Dot blot result of Phospho-Histone H3 (Ser10) Recombinant Rabbit mAb
Lane 1: Histone H3 phospho S10 peptide
Lane 2: Histone H3 phospho S10 peptide
Lane 3: Histone H3 unmodified peptide
Primary antibody: Phospho-Histone H3 (Ser10) Recombinant Rabbit mAb at 1/10000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
ChIP
Chromatin immunoprecipitation (ChIP) was performed on HeLa + Nocodazole (100ng/ml,18h) (+) (left) and HeLa + Nocodazole (100ng/ml,18h) (-) (right) cells
cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used Phospho-Histone H3 (Ser10) Recombinant Rabbit mAb (S-960-58) and Rabbit mAb IgG
Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
qPCR (%input: immunoprecipitated DNA / input DNA)
showed the enrichment of RPL30 and GAPDH in Phospho-
Histone H3(Ser10) Recombinant Rabbit mAb (S-960-58)-
immunoprecipitated sample.
