WB result of Phospho-EGFR (Tyr1068) Recombinant Rabbit mAb
Primary antibody: Phospho-EGFR (Tyr1173) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated A431 whole cell lysate 20 µg
Lane 2: A431 treated with 200 ng/ml EGF for 10 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 134 kDa
Observed MW: 180 kDa
This blot was developed with high sensitivity substrate
Phospho-EGFR (Tyr1068) Recombinant Rabbit mAb (S-1189-101)
Phospho-EGFR (Tyr1068) Recombinant Rabbit mAb (S-1189-101)
Price:
Regular price
$130.00 SGD
Regular price
Sale price
$130.00 SGD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-EGFR (Tyr1068) |
| Synonyms | Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; ERBB; ERBB1; HER1; EGFR |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cell membrane |
| Accession | P00533 |
| Clone Number | S-1189-101 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu |
| Positive Sample | A431 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu |
| ICC | 1:500 | Hu |
Background
Phospho-EGFR (Tyr1068) is the phosphorylated form of the epidermal growth factor receptor (EGFR) at the tyrosine residue 1068, indicating the activated state of EGFR. Upon binding of EGFR to its ligands (e.g., EGF), the receptor dimerizes and undergoes autophosphorylation, with phosphorylation at Tyr1068 being one of the key activation events. This modification provides a binding site for downstream signaling molecules (e.g., Grb2 and PI3K), thereby initiating signaling pathways such as MAPK/ERK and PI3K/AKT, which regulate cell proliferation, survival, migration, and differentiation. Abnormal phosphorylation at Tyr1068 is closely associated with the development, progression, and treatment resistance of various cancers (e.g., non-small cell lung cancer, colorectal cancer), making it an important biomarker for cancer diagnosis and targeted therapy.
Picture
Picture
Western Blot
Dot Blot
Dot blot result of Phospho-EGFR (Tyr1068) Recombinant Rabbit mAb
Lane 1: EGFR (Tyr1068) phospho peptide
Lane 2: EGFR (Tyr1068) unmodified peptide
Primary antibody: Phospho-EGFR (Tyr1068) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Immunocytochemistry
ICC analysis of A431 cells treated with EGF (200 ng/mL for 10 min) (top panel) and A431 cells untreated with EGF (200 ng/mL for 10 min) (below panel). Anti-××× antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
