WB result of NRGN Recombinant Rabbit mAb
Primary antibody: NRGN Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse lung lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Negative control: mouse lung lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 8 kDa
Observed MW: 15 kDa
NRGN Recombinant Rabbit mAb (S-1624-60)
NRGN Recombinant Rabbit mAb (S-1624-60)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | NRGN |
| Synonyms | Neurogranin; Ng; RC3 |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm |
| Accession | Q92686 |
| Clone Number | S-1624-60 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | Mouse brain, Rat brain |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms, Rt |
| IHC-P | 1:1000 | Hu, Ms, Rt |
| IF | 1:2000 | Hu |
Background
NRGN, or neurogranin, is a brain-specific protein found predominantly in telencephalic neurons. It is known to be a postsynaptic protein kinase substrate that binds calmodulin in the absence of calcium. NRGN is also under the control of thyroid hormone in specific neuronal subsets, playing a role in the mental states associated with hypothyroidism during development and in adult subjects. The gene is located on chromosome 11q24 and contains four exons and three introns, with exons 1 and 2 encoding the protein. NRGN is considered a direct target for thyroid hormone in the human brain, and its expression is crucial for understanding the impact of thyroid function on cognitive and mental health.
Picture
Picture
Western Blot
WB result of NRGN Recombinant Rabbit mAb
Primary antibody: NRGN Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat lung lysate 20 µg
Lane 2: rat brain lysate 20 µg
Negative control: rat lung lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 8 kDa
Observed MW: 15 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-NRGN antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows positive staining in paraffin-embedded human liver. Anti-NRGN antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-NRGN antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-NRGN antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded human cerebral cortex. Anti-NRGN antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
