WB result of Non-phospho (Active) β-Catenin (Ser37/Thr41) Recombinant Rabbit mAb
Primary antibody: Non-phospho (Active) β-Catenin (Ser37/Thr41) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 85 kDa
Observed MW: 70-100 kDa
Non-phospho (Active) β-Catenin (Ser37/Thr41) Recombinant Rabbit mAb (S-1813-42)
Non-phospho (Active) β-Catenin (Ser37/Thr41) Recombinant Rabbit mAb (S-1813-42)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Non-phospho (Active) β-Catenin (Ser37/Thr41) |
| Synonyms | CTNNB1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus, Cell membrane |
| Accession | P35222 |
| Clone Number | S-1813-42 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms |
| Positive Sample | HeLa, 293T, A431, NIH/3T3 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu, Ms |
| IHC-P | 1:500 | Hu |
Background
Non-phospho (Active) β-Catenin (Ser37/Thr41) refers to a form of the β-catenin protein that is not phosphorylated at the serine 37 and threonine 41 residues. This unphosphorylated state stabilizes β-catenin, allowing it to accumulate in the cytoplasm and subsequently translocate to the nucleus, where it acts as a transcriptional co-activator in the Wnt signaling pathway. The presence of non-phospho β-Catenin (Ser37/Thr41) is often associated with active Wnt signaling, which plays a critical role in cell proliferation, differentiation, and development. Detection of this form is commonly used in research to assess Wnt pathway activity.
Picture
Picture
Western Blot
WB result of Non-phospho (Active) β-Catenin (Ser37/Thr41) Recombinant Rabbit mAb
Primary antibody: Non-phospho (Active) β-Catenin (Ser37/Thr41) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 85 kDa
Observed MW: 100 kDa
Dot Blot
Dot blot result of Non-phospho (Active) β-Catenin (Ser37/Thr41) Recombinant Rabbit mAb
Lane 1: Non-phospho β-Catenin (Ser33/37/Thr41) peptide
Lane 2: Phospho β-Catenin (Ser33) peptide
Lane 3: Phospho β-Catenin (Ser37) peptide
Lane 4: Phospho β-Catenin (Thr41) peptide
Primary antibody: Non-phospho (Active) β-Catenin (Ser37/Thr41) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti- Non-phospho (Active) β-Catenin (Ser37/Thr41) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti- Non-phospho (Active) β-Catenin (Ser37/Thr41) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti- Non-phospho (Active) β-Catenin (Ser37/Thr41) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti- Non-phospho (Active) β-Catenin (Ser37/Thr41) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
