WB result of Myosin light chain 3 Rabbit mAb
Primary antibody: Myosin light chain 3 Rabbit mAb at 1/1000 dilution
Lane 1: mouse heart lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 24 kDa
Myosin light chain 3 Recombinant Rabbit mAb (S-R277)
Myosin light chain 3 Recombinant Rabbit mAb (S-R277)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Myosin light chain 3 |
| Synonyms | Cardiac myosin light chain 1 (CMLC1), Myosin light chain 1 slow-twitch muscle B/ventricular isoform (MLC1SB), Ventricular myosin alkali light chain, Ventricular myosin light chain (VLCl), Ventricular/slow twitch myosin alkali light chain (MLC-lV/sb), MYL3 |
| Location | Cytosol |
| Accession | P08590 |
| Clone Number | S-R277 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | null |
Background
Myosin essential light chain (ELC), ventricular/cardiac isoform is a protein that in humans is encoded by the MYL3 gene. This cardiac ventricular/slow skeletal ELC isoform is distinct from that expressed in fast skeletal muscle (MYL1) and cardiac atrial muscle (MYL4). Ventricular ELC is part of the myosin molecule and is important in modulating cardiac muscle contraction. Mutations in MYL3 have been identified as a cause of familial hypertrophic cardiomyopathy, and associated with a mid-left ventricular chamber type hypertrophy.
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Western Blot
WB result of Myosin light chain 3 Rabbit mAb
Primary antibody: Myosin light chain 3 Rabbit mAb at 1/1000 dilution
Lane 1: rat heart lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 22 kDa
