WB result of HVEM/TNFRSF14 Rabbit mAb
Primary antibody: HVEM/TNFRSF14 Rabbit mAb at 1/1000 dilution
Lane 1: recombinant human HVEM/TNFRSF14 protein 10 ng
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 20 kDa
Observed MW: 27, 38 kDa
(This blot was developed with high sensitivity substrate)
HVEM/TNFRSF14 Recombinant Rabbit mAb (S-727-70)
HVEM/TNFRSF14 Recombinant Rabbit mAb (S-727-70)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Synonyms | Tumor necrosis factor receptor superfamily member 14, Herpes virus entry mediator A, HveA, Tumor necrosis factor receptor-like 2, TR2, CD270, HVEA, HVEM |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | Q92956 |
| Clone Number | S-727-70 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC, FCM |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | null |
| ICC | 1:500 | null |
| FCM | 1:50 | null |
Background
Herpesvirus entry mediator (HVEM), also known as tumor necrosis factor receptor superfamily member 14 (TNFRSF14), is a human cell surface receptor of the TNF-receptor superfamily encoded by the TNFRSF14 gene. Mutations in this protein have been recurrently been associated to cases of diffuse large B-cell lymphoma and pediatric-type follicular lymphoma. This receptor was identified as a cellular mediator of herpes simplex virus (HSV) entry. Binding of HSV viral envelope glycoprotein D (gD) to this receptor protein has been shown to be part of the viral entry mechanism.
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Western Blot
FC
Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) labelling HVEM/TNFRSF14 antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
Immunocytochemistry
ICC shows positive staining in Ramos cells. Anti-HVEM/TNFRSF14 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
Negative control: ICC shows negative staining in 293T cells. Anti-HVEM/TNFRSF14 antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
