Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. The EC50 for this effect is less than 150ng/ml.
E-Selectin/CD62E Fc Chimera Protein, Mouse
E-Selectin/CD62E Fc Chimera Protein, Mouse
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$256 USD
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Product Details
Product Details
Product Specification
| Species | Mouse |
| Synonyms | SELE, RP1-117P20.2, ELAM1, ESEL, LECAM2 |
| Accession | Q00690 |
| Amino Acid Sequence | Trp22-Pro557, with C-terminal Fc |
| Expression System | HEK293 |
| Molecular Weight | 95-125kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE & RP-HPLC |
| Conjugation | Unconjugated |
| Tag | Human Fc Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, PH7.4, 5% trehalose |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1.Matsumoto K, et al. (2010) Soluble adhesion molecule E-selectin predicts cardiovascular events in Japanese patients with type 2 diabetes mellitus. Metabolism. 59(3): 320-4. |
Background
E-selectin, also known as endothelial leukocyte adhesion molecule-1 (ELAM-1) and CD62E, is an inducible adhesion molecule that is expressed on the surfaces of stimulated vascular endothelial cells. It is sometimes implicated in cancer cell metastasis. E-selectin features a complex mosaic structure, which includes a large extracellular region composed of a lectin domain, an EGF-like domain, and a short consensus repeat (SCR) domain. This is followed by a transmembrane region and a relatively short cytoplasmic tail of 32 amino acids (aa).
As a member of the selectin family, E-selectin recognizes and binds to sialylated carbohydrates, including those belonging to the Lewis X and Lewis A families, which are found on the surface of monocytes, granulocytes, and T-lymphocytes. It supports the rolling and stable arrest of leukocytes on activated vascular endothelium. Additionally, E-selectin has been shown to transduce an activating stimulus via the mitogen-activated protein kinase (MAPK) cascade into the endothelial cell during leukocyte adhesion.
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Bioactivity
SDS-PAGE
1μg (R: reducing condition, N: non-reducing condition).
RP-HPLC
The purity of E-Selectin/CD62E Fc Chimera Protein, Mouse is more than 95% determined by RP-HPLC.
