ICC shows positive staining in HeLa cells. Anti-DNA:RNA hybrid antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
DNA:RNA hybrid Recombinant Mouse mAb (S-R180)
DNA:RNA hybrid Recombinant Mouse mAb (S-R180)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Location | Cytoplasm, Nucleus |
| Clone Number | S-R180 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG2a |
| Application | ICC |
| Reactivity | Hu, Ms |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20°C as supplied |
Dilution
| application | dilution | species |
| ICC | 1:500 | null |
Background
RNA:DNA hybrids serve as primers for DNA replication, regulate gene expression and are structural components of specific eukaryotic genomic elements, such as telomeres, centromeres and ribosomal DNA. RNA:DNA hybridization is also a crucial step in cell-specific processes, such as immunoglobulin class-switch recombination (CSR) in activated B cells and CRISPR/Cas9-mediated adaptive immunization in prokaryotic cells.
Picture
Picture
Immunocytochemistry
ICC shows positive staining in NIH/3T3 cells. Anti-DNA:RNA hybrid antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
