The DDB1/CRBN Complex Flag&His Tag Protein, Human activity was detected using HTRF technology. The reaction was performed by incubating the Human GSPT1, GST tag Protein, DDB1/CRBN Complex Flag&His Tag Protein, Human and varying concentration of CC-885 and antibody at 25℃ for 120 min, then reading ratio 665/620 on BMG.
DDB1/CRBN Complex, Flag&His Tag, Human
DDB1/CRBN Complex, Flag&His Tag, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | DDB1/CRBN Complex |
| Accession | Q16531、Q96SW2 |
| Amino Acid Sequence | DDB1 Flag Tag, Human |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | 128&53kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tags & Cleavage sites | Flag Tag; His Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 20mM Tris,300mM NaCl,10%Glycerol,1mM DTT, pH7.5. |
| Stability & Storage | ·12 months from date of receipt, -20 to -70 °C as supplied. |
| Reference | Fischer E.S.,et al. (2014) Nature 512: 49 |
Background
Cereblon (CRBN) is the substrate recognition component of DCX (DDB1-CUL4-X-box) E3 Ubiquitin ligase complexes that mediate the ubiquitination of numerous target proteins including the transcriptional regulator MEIS2. CRBN plays an important role in limb outgrowth, and its function in this process is perturbed by the binding of thalidomide and related compounds. Binding of CRBN to Cullin-4 is mediated in part by DNA damage-binding protein 1 (DDB1), a component of multiple DCX class ligases. In addition to its role in limb development, DDB1 is also involved in various DNA damage response mechanisms. This product consists of an untagged complex of DDB1 and CRBN.
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Bioactivity
SDS-PAGE
1μg (R: reducing condition, N: non-reducing condition).
