WB result of Clathrin heavy chain Recombinant Rabbit mAb
Primary antibody: Clathrin heavy chain Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: SH-SY5Y whole cell lysate 20 µg
Lane 4: 293T whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 192 kDa
Observed MW: 192 kDa
Clathrin heavy chain Recombinant Rabbit mAb (S-1690-25)
Clathrin heavy chain Recombinant Rabbit mAb (S-1690-25)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Clathrin heavy chain |
| Synonyms | Clathrin heavy chain 1; Clathrin heavy chain on chromosome 17 (CLH-17); CLH17; CLTCL2; KIAA0034; CLTC |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cytoskeleton |
| Accession | Q00610 |
| Clone Number | S-1690-25 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, A431, SH-SY5Y, 293T, NIH/3T3, Neuro-2a, PC-12, C6 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:1000 | Hu, Ms, Rt |
Background
The clathrin heavy chain (Chc) is a crucial component of the clathrin protein complex, which plays a central role in clathrin-mediated endocytosis, a fundamental cellular process for internalizing various cargoes such as membrane proteins, nutrients, and even pathogens. With a molecular weight of approximately 190 kDa, the clathrin heavy chain forms a triskelion structure along with three clathrin light chains, providing the structural backbone of the clathrin lattice. This lattice can form cages of different sizes and shapes, essential for the invagination and excision of vesicles during endocytosis. The clathrin heavy chain contains 145-residue repeats known as Clathrin Heavy Chain Repeats, which fold into elongated superhelical structures and define distinct domains, including the proximal, knee, distal, and ankle domains. Its N-terminal domain (NTD) adopts a WD40 beta-propeller structure and is vital for interacting with various adaptor proteins (APs) that link the clathrin scaffold to the membrane. These interactions are mediated through multiple binding sites, or "boxes," on the NTD, including the Clathrin box, W-box, and Arrestin box, each recognizing specific motifs on the adaptor proteins.
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Western Blot
WB result of Clathrin heavy chain Recombinant Rabbit mAb
Primary antibody: Clathrin heavy chain Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: Neuro-2a whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 192 kDa
Observed MW: 192 kDa
WB result of Clathrin heavy chain Recombinant Rabbit mAb
Primary antibody: Clathrin heavy chain Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 192 kDa
Observed MW: 192 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-Clathrin heavy chain antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-Clathrin heavy chain antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-Clathrin heavy chain antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Clathrin heavy chain antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Clathrin heavy chain antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-Clathrin heavy chain antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Clathrin heavy chain antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-Clathrin heavy chain antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
