WB result of CHMP2B Recombinant Rabbit mAb
Primary antibody: CHMP2B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: 293T whole cell lysate 20 µg
Lane 5: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 24 kDa
Observed MW: 27 kDa
CHMP2B Recombinant Rabbit mAb (S-1639-20)
CHMP2B Recombinant Rabbit mAb (S-1639-20)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CHMP2B |
| Synonyms | Charged multivesicular body protein 2b; CHMP2.5; Chromatin-modifying protein 2b (CHMP2b); Vacuolar protein sorting-associated protein 2-2 (Vps2-2; hVps2-2) |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | Q9UQN3 |
| Clone Number | S-1639-20 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, A549, A431, 293T, HepG2, mouse brain, rat brain |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
CHMP2B is a component of the ESCRT-III complex, which plays a critical role in membrane remodeling and the regulation of endosomal trafficking, particularly in the degradation of endosomes into lysosomes. It is associated with the cytoskeleton in neurons and is thought to strengthen dendritic spines. Mutations in the CHMP2B gene are linked to autosomal dominant frontal temporal dementia (FTD) in humans, and are associated with the death of cortical neurons due to dendritic retraction and the accumulation of ubiquitinated proteins and autophagosomes. CHMP2B also has a role in regulating TDP-43 phosphorylation and cytotoxicity independent of autophagy via the protein kinase CK1. Furthermore, CHMP2B's function in the autophagy pathway and its impact on TDP-43 phosphorylation can independently contribute to cell toxicity and neurodegeneration. The protein's role in membrane remodeling is highlighted by its ability to form a protein network on the membrane surface, enhancing membrane rigidity, which is important for understanding the mechanics of membrane remodeling and scission.
Picture
Picture
Western Blot
WB result of CHMP2B Recombinant Rabbit mAb
Primary antibody: CHMP2B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 24 kDa
Observed MW: 27 kDa
WB result of CHMP2B Recombinant Rabbit mAb
Primary antibody: CHMP2B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 24 kDa
Observed MW: 27 kDa
Immunocytochemistry
ICC shows positive staining in HepG2 cells. Anti- CHMP2B antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
