Flow cytometric analysis of mouse primary splenocytes labeling CD8α at 1/2000 dilution (right panel) compared with a Rat IgG, monoclonal Isotype Control (left panel). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody.
Then cells were stained with CD4 - Alexa Fluor® 647 separately. CD4 and CD8α are mutually exclusive expressed in mouse primary splenocytes. Gated on total viable cells.
CD8α Rat mAb (S-353-45)
CD8α Rat mAb (S-353-45)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD8α |
| Synonyms | T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2, Lyt-2, Lyt2 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P01731 |
| Clone Number | S-353-45 |
| Antibody Type | Rat mAb |
| Isotype | IgG1 |
| Application | FCM |
| Reactivity | Ms |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1:2000 | null |
Background
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen-presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
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