WB result of CD49f Rabbit mAb
Primary antibody: CD49f Rabbit mAb at 1/1000 dilution
Lane 1: SW480 whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 127 kDa
Observed MW: 127 kDa
CD49f Recombinant Rabbit mAb (S-1019-73)
CD49f Recombinant Rabbit mAb (S-1019-73)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Synonyms | Integrin alpha-6, CD49 antigen-like family member F, VLA-6, ITGA6 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | P23229 |
| Clone Number | S-1019-73 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IHC-P | 1:100 |
Background
CD49f protein, also known as integrin-6 (ITGA6) or integrin alpha-6 chain protein, is a type I transmembrane glycoprotein belonging to the α-subfamily. CD49f may have two distinct cytoplasmic domains resulting from alternative splicing variants of mRNA, α6A and α6B, which exhibit different stem-like functions. Among them, the α6B variant is closely related to the cancer stem cell (CSC) phenotype. CD49f plays a crucial role in cell adhesion to the extracellular matrix. It typically forms heterodimers with integrin β1 and β4, serving as a receptor for various laminin proteins. Research has shown that the heterodimerization of CD49f interacts with signaling pathways, inducing stem-like and invasive characteristics in breast cancer cells. Moreover, CD49f is not only a stemness-related gene that can serve as a surface marker for various stem cells, such as embryonic stem cells, neural stem cells, and hematopoietic stem cells, but also plays a significant role in maintaining stem cell self-renewal and stemness through its interaction with ECM components (e.g., laminin) in the microenvironment. Previous studies have also found that the adhesion molecule CD49f is differentially expressed in fetal and adult bone marrow-derived mesenchymal stem cells (BMSCs), and the interaction between stem cells and their supporting cells or ECM in the microenvironment mediated by CD49f is crucial for maintaining stemness.
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Western Blot
WB result of CD49f Rabbit mAb
Primary antibody: CD49f Rabbit mAb at 1/1000 dilution
Lane 1: mouse lung lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 127 kDa
Observed MW: 127 kDa
WB result of CD49f Rabbit mAb
Primary antibody: CD49f Rabbit mAb at 1/1000 dilution
Lane 1: rat stomach lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 127 kDa
Observed MW: 127 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-CD49f antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta. Anti-CD49f antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-CD49f antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CD49f antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-CD49f antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-CD49f antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
