Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) labelling CD45RA antibody at 1/1000 (0.1 μg) dilution (Right) compared with a Mouse monoclonal IgG isotype control (Left). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Gated on total viable cells.
CD45RA Recombinant Mouse mAb (S-R377)
CD45RA Recombinant Mouse mAb (S-R377)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Location | Cell membrane |
| Accession | P08575 |
| Clone Number | S-R377 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG2b,k |
| Application | IHC-P, IF |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:1000 | |
| IF | 1:500 |
Background
CD45RA is an isoform of CD45 (leukocyte common antigen), with a molecular weight of approximately 220kDa or 180kDa (the specific value may vary depending on different studies or measurement methods). This protein is primarily expressed on B cells, monocytes, and a small subset of T cells. In T cells, CD45RA plays a crucial role in regulating immune receptor signaling pathways, modulating T cell activation and suppression. In NK cells, CD45RA is essential for the exertion of cytotoxicity. In the thymus, the expression of CD45RA increases as T cells mature, regulating the transition from naive T cells to mature T cells. Additionally, the protein encoded by the CD45RA gene possesses dephosphorylating activity, which can affect the proliferation, differentiation, and activation of immune cells. In medical applications, CD45RA is used in combination with other B-cell antibodies for the study of B-cell lymphomas. It also plays a key role in the diagnosis of diseases such as acute lymphocytic leukemia and aplastic anemia.
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Immunohistochemistry
IHC shows positive staining in paraffin-embedded human spleen. Anti-CD45RA antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thymus. Anti-CD45RA antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD45RA antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-CD45RA antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human mantle cell lymphoma. Anti-CD45RA antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human diffuse large B-cell lymphoma. Anti-CD45RA antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human anaplastic large cell lymphoma. Anti-CD45RA antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded human tonsil. Anti-CD45RA antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat Anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) (Alexa Fluor® 488 Conjugate) (S0B4052) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human anaplastic large cell lymphoma. Anti-CD45RA antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat Anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) (Alexa Fluor® 488 Conjugate) (S0B4052) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human diffuse large B cell lymphoma. Anti-CD45RA antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat Anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) (Alexa Fluor® 488 Conjugate) (S0B4052) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
