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Bovine bPAG1, His tag

Bovine bPAG1, His tag

Catalog Number: S0A9004 Brand: Starter
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Regular price $70 USD
Regular price Sale price $70 USD
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Product Details

Product Specification


Species Bovine
Synonyms Pregnancy-specific protein B (PSP-B)
Accession Q29432
Amino Acid Sequence

Protein sequence(Q29432, Arg54-Val380, with C-10*His)
RGSNLTTHPLRNIKDLVYMGNITIGTPPQEFQVVFDTASSDLWVPSDFCTSPACSTHVRFRHLQSSTFRLTNKTFRITYGSGRMKGVVVHDTVRIGNLVSTDQPFGLSIEEYGFEGRIYDGVLGLNYPNISFSGAIPIFDKLKNQRAISEPVFAFYLSKDEREGSVVMFGGVDHRYYEGELNWVPLIQAGDWSVHMDRISIERKIIACSDGCKALVDTGTSDIVGPRRLVNNIHRLIGAIPRGSEHYVPCSEVNTLPSIVFTINGINYPVPGRAYILKDDRGRCYTTFQENRVSSSTETWYLGDVFLRLYFSVFDRGNDRIGLARAVGGGGSHHHHHHHHHH

Expression System HEK293
Molecular Weight Theoretical:38.4kDa Actual:50kDa
Purity

>95% by SDS-PAGE

Endotoxin <1EU/μg
Tag His Tag
Physical Appearance Lyophilized Powder
Storage Buffer Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, 1mM EDTA, pH7.4.
Reconstitution Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.
Stability & Storage

12 months from date of receipt, -20 to -70 °C as supplied. 6 months, -20 to -70 °C under sterile conditions after reconstitution. 1 week, 2 to 8 °C under sterile conditions after reconstitution. Please avoid repeated freeze-thaw cycles.

Background

PAG constitute a group of pregnancy-specific proteins secreted by the chorionic epithelium (trophectoderm) of the placenta. The proteins whose cDNA sequences have so far been identified are bovine and ovine PAG1, bovine PAG2, porcine PAG1 and PAG2, and equine PAG. bPAG1, also known as pregnancy-specific protein B, becomes detectable in maternal blood soon after implantation is initiated and can be used to diagnose pregnancy. The synthesis of bPAG1 is localized to a subpopulation of binucleate cells within the trophectoderm , which probably have an endocrine function, since they possess secretory granules that contain the hormone placental lactogen and PAG1. As implantation is initiated these binucleate cells migrate from the trophectoderm and fuse with uterine epithelial cells. They then release the contents of their secretory granules directly into the maternal system.

Picture

SDS-PAGE

2μg(R: reducing conditions)

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