Flow cytometric analysis of C57BL/6 mouse splenocytes labelling Armenian hamster Anti-Mouse TCR β Antibody at 1/200 (1 μg) dilution Goat Anti-Armenian hamster IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD8 - Alexa Fluor® 647 (Right panel) or CD4 - Brilliant Violet 421™ (Left panel) antibody separately.
Product Details
Product Details
Product Specification
Host | Armenian hamster |
Antigen | Mouse TCR β |
Synonyms | T-cell receptor beta-1 chain C region |
Location | Membrane |
Accession | P01852 |
Clone Number | S-R616 |
Antibody Type | Recombinant mAb |
Isotype | IgG |
Application | FCM |
Reactivity | Ms |
Positive Sample | C57BL/6 mouse splenocytes |
Purification | Protein G |
Concentration | 2 mg/ml |
Conjugation | Unconjugated |
Physical Appearance | Liquid |
Storage Buffer | PBS pH7.4 |
Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
application | dilution | species |
FCM | 1:200 | Ms |
Background
The TCR β (T-cell receptor beta chain) is a crucial component of the T-cell receptor, which, together with the TCR α chain, forms the αβTCR on the surface of T cells. This receptor is responsible for recognizing and binding to antigenic peptide complexes presented by MHC molecules. The diversity of the TCR β chain is generated through a combination of random recombination of multiple germline gene segments, gene rearrangement, junctional diversity, N-region nucleotide insertions, and random pairing of α and β chains. These mechanisms collectively provide the TCR repertoire with immense diversity, enabling T cells to recognize a wide range of antigens.
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