WB result of APE Rabbit mAb
Primary antibody: APE Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 35 kDa
Observed MW: 37 kDa
APE Recombinant Rabbit mAb (S-R349)
APE Recombinant Rabbit mAb (S-R349)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Synonyms | DNA-(apurinic or apyrimidinic site) endonuclease, APEX nuclease (APEN), Apurinic-apyrimidinic endonuclease 1 (AP endonuclease 1; APE-1), REF-1, Redox factor-1, APE, APE1, APEX, APX, HAP1, Apex1 |
| Location | Nucleus |
| Accession | P27695 |
| Clone Number | S-R349 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | null |
| IHC-P | 1:500 | null |
Background
Mammalian apurinic/apyrimidinic endonuclease 1 (APEX1) is a small pleiotropic protein, which is composed of 2 distinct structural catalytic domains. The catalytic C-terminal region of APEX1 exhibits different nucleic acid enzymatic activities. Apurinic/apyrimidinic (AP) endonuclease activity is the predominant nuclease function, with a central role in the base excision repair (BER) pathway. Aside from its canonical function in DNA repair, the APEX1 nuclease domain is also implicated in transcriptional and post-translational regulation. The catalytic N-terminal protein domain of APEX1, known as redox effector function (REF-1), controls the DNA binding activity of multiple transcription factors (TFs) through modulation of their redox status.
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Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-APE antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-APE antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-APE antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human bladder cancer. Anti-APE antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-APE antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-APE antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-APE antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
