WB result of ARNT Recombinant Rabbit mAb
Primary antibody: ARNT Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: MDA-MB-231 whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Lane 5: 293T whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 87 kDa
Observed MW: 85 kDa
ARNT Recombinant Rabbit mAb (S-2263-43)
ARNT Recombinant Rabbit mAb (S-2263-43)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ARNT |
| Synonyms | Aryl hydrocarbon receptor nuclear translocator; ARNT protein; Class E basic helix-loop-helix protein 2 (bHLHe2); Dioxin receptor; nuclear translocator; Hypoxia-inducible factor 1-beta (HIF-1-beta; HIF1-beta); BHLHE2 |
| Immunogen | Recombinant Protein |
| Location | Nucleus |
| Accession | P27540 |
| Clone Number | S-2263-43 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HepG2, MDA-MB-231, MCF7, HeLa, 293T, NIH/3T3, RAW264.7, PC-12 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:10000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator) protein is a crucial transcription factor involved in various cellular processes. It forms heterodimers with other proteins such as the aryl hydrocarbon receptor (AhR), playing a key role in the regulation of gene expression in response to environmental toxins and endogenous signals. ARNT is essential for the proper functioning of the AhR pathway, which is involved in xenobiotic metabolism, cell differentiation, and immune response modulation. Additionally, ARNT has been implicated in developmental processes and maintaining cellular homeostasis. Dysregulation of ARNT function has been associated with several pathological conditions, highlighting its importance in both normal physiology and disease.
Picture
Picture
Western Blot
WB result of ARNT Recombinant Rabbit mAb
Primary antibody: ARNT Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: RAW264.7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 87 kDa
Observed MW: 85 kDa
WB result of ARNT Recombinant Rabbit mAb
Primary antibody: ARNT Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 87 kDa
Observed MW: 85 kDa
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- ARNT antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
