ARNT Recombinant Rabbit mAb (S-2263-43)

Catalog Number: S0B6110 Application: WB, ICC, ChIP Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	ARNT
Synonyms	Aryl hydrocarbon receptor nuclear translocator; ARNT protein; Class E basic helix-loop-helix protein 2 (bHLHe2); Dioxin receptor; nuclear translocator; Hypoxia-inducible factor 1-beta (HIF-1-beta; HIF1-beta); BHLHE2
Immunogen	Recombinant Protein
Location	Nucleus
Accession	P27540
Clone Number	S-2263-43
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, ICC, ChIP
Reactivity	Hu, Ms, Rt
Positive Sample	HepG2, MDA-MB-231, MCF7, HeLa, 293T, NIH/3T3, RAW264.7, PC-12
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:10000	Hu, Ms, Rt
ICC	1:500	Hu
ChIP	1:20-1:50	Hu

Background

ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator) protein is a crucial transcription factor involved in various cellular processes. It forms heterodimers with other proteins such as the aryl hydrocarbon receptor (AhR), playing a key role in the regulation of gene expression in response to environmental toxins and endogenous signals. ARNT is essential for the proper functioning of the AhR pathway, which is involved in xenobiotic metabolism, cell differentiation, and immune response modulation. Additionally, ARNT has been implicated in developmental processes and maintaining cellular homeostasis. Dysregulation of ARNT function has been associated with several pathological conditions, highlighting its importance in both normal physiology and disease.

Picture

Western Blot

WB result of ARNT Recombinant Rabbit mAb
Primary antibody: ARNT Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: MDA-MB-231 whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Lane 5: 293T whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 87 kDa
Observed MW: 85 kDa

WB result of ARNT Recombinant Rabbit mAb
Primary antibody: ARNT Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: RAW264.7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 87 kDa
Observed MW: 85 kDa

WB result of ARNT Recombinant Rabbit mAb
Primary antibody: ARNT Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 87 kDa
Observed MW: 85 kDa

Immunocytochemistry

ICC shows positive staining in HeLa cells. Anti- ARNT antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

ChIP

Chromatin immunoprecipitation (ChIP) was performed on HepG2 cells cross-linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used ARNT Recombinant Rabbit mAb (S-2263-43) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
Post -immunoprecipitation, both samples were washed, eluted, and cross-links reversed. Purified DNA was analyzed by qPCR.
qPCR showed the enrichment of CYP1A1, NFE2L2 and SAT-α in ARNT Recombinant
Rabbit mAb (S-2263-43)-immunoprecipitated sample.