Glycoprotein Cleaving Enzyme Products

Glycoprotein cleavage enzyme products are mainly used to cleave sugar chains in glycoproteins and reveal glycosylation patterns, which are crucial for studying sugar chain structure and function.

PNGase F:

PNGase F is an amidase that can cleave high mannose, hybrid and complex oligosaccharides from N-glycoproteins. It is involved in protein degradation, organ development, individual growth and other processes in the body. In research, PNGase F is used to remove almost all N-linked oligosaccharides, and can work under native or denaturing conditions, providing a basis for further sugar chain analysis.

Endo S:

Endo S is a highly specific endoglycosidase that cleaves N-linked sugars from between the chitobiose core structures of IgG heavy chains. Endo S is very useful for studying the glycosylation pattern of IgG and its effect on antibody function.

Endo H:

Endo H can cleave the chitobiose core structure of high mannose in N-glycoproteins and some hybrid oligosaccharides, removing N-linked high mannose in glycoproteins. It is used in research to analyze the glycosylation pattern of glycoproteins, especially when studying high mannose glycans.

Endo S2:

Endo S2 can cleave the glycosidic bond between the two innermost N-acetylglucosamines of the N-linked sugar chain of glycoproteins, and is effective for high mannose, hybrid and biantennary complex sugar chains. This property makes it very useful in sugar chain analysis and structural characterization, especially in studying the effect of sugar chain structure on protein function.