Determined by its ability to inhibit the proliferation of murine WEHI-279 cells. The expected EC50 is 0.034-0.12 ng/ml.
IFN-γ Protein, Mouse
IFN-γ Protein, Mouse
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Product Details
Product Details
Product Specification
| Species | Mouse |
| Synonyms | IFN-γ, Immune Interferon, type II interferon, T cell interferon, MAF, IFNG, IFG, IFI, IFN-gamma |
| Accession | P01580 |
| Amino Acid Sequence | His23-Cys155 |
| Expression System | E.coli |
| Molecular Weight | 13 kDa (Reducing) |
| Purity | >95% by SDS-PAGE & HPLC. |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 20 mM Tris-HCl pH8.0, 300 mM NaCl, 5 % trehalose, 0.05 % Tween-20. |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.·3 months, -20 to -80℃ under sterile conditions after reconstitution.·1 week, 2 to 8℃ under sterile conditions after reconstitution.·Please avoid repeated freeze-thaw cycles. |
| Reference | Nat Methods. 2008 Dec;5(12):1011-7. |
Background
Interferon gamma (IFN-γ) is a cytokine critical to both innate and adaptive immunity, and functions as the primary activator of macrophages, in addition to stimulating natural killer cells and neutrophils. A non-IgE-mediated anaphylactic reaction and severe bronchospasm have been reported once after the first injection of interferon gamma. IFN-γ activates cells via a different receptor than IFN-α and IFN-β, which accounts for the different physiological properties of the proteins. Production of IFN-γ is largely restricted to activated CD4+ TH1 T cells, CD8+ T cells, and natural killer cells. One of the most important consequences of IFN-γ secretion is the activation of macrophages. In addition, IFN-γ plays a central role in inflammatory responses by activating endothelial cells, promoting TH1 cell development and cellular immune responses, and up-regulation of major histocompatability complex protein expression on antigen-presenting cells.
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Bioactivity
SDS-PAGE
2μg (R: reducing condition, N: non-reducing condition).
RP-HPLC
>95% as determined by RP-HPLC.
