WB result of ROCK1+ROCK2 Recombinant Rabbit mAb
Primary antibody: ROCK1+ROCK2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 158 kDa
Observed MW: 160 kDa
ROCK1+ROCK2 Recombinant Rabbit mAb (S-1305-26)
ROCK1+ROCK2 Recombinant Rabbit mAb (S-1305-26)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ROCK1+ROCK2 |
| Synonyms | Rho-associated protein kinase 1, Renal carcinoma antigen NY-REN-35, Rho-associated coiled-coil-containing protein kinase 1, Rho-associated coiled-coil-containing protein kinase I (ROCK-I), p160 ROCK-1 (p160ROCK), Rho-associated protein kinase 2, Rho kinase 2, Rho-associated, coiled-coil-containing protein kinase 2, Rho-associated, coiled-coil-containing protein kinase II (ROCK-II), p164 ROCK-2 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | Q13464、 O75116 |
| Clone Number | S-1305-26 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC, ICFCM, IP |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Cz |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IP | 1:50 | |
| ICC | 1:500 | |
| ICFCM | 1:50 |
Background
Rho-associated protein kinase (ROCK), also known as Rho-kinase, is a serine/threonine protein kinase that acts as a key downstream effector of the small GTPase RhoA. Once activated, ROCK can influence a multitude of cellular functions, such as the formation of actin filaments through the activation of LIM-kinase 2 (LIMK2), leading to increased actin polymerization. Additionally, ROCK is involved in smooth muscle contraction by inactivating myosin light chain phosphatase 1 (MYPT-1) through phosphorylation, thereby enhancing the phosphorylation of myosin light chains. ROCK has been implicated in a wide range of diseases, including pulmonary hypertension, coronary heart disease, respiratory disease, ocular disease, gastrointestinal disorders, and cancer. In the context of cancer, ROCK is believed to promote tumor progression by enhancing cell migration, invasion, and angiogenesis. Furthermore, ROCK signaling is also involved in the regulation of immune responses to viral infections and has been identified as a potential target for the development of novel antiviral therapeutics.
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Western Blot
WB result of ROCK1+ROCK2 Recombinant Rabbit mAb
Primary antibody: ROCK1+ROCK2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 158 kDa
Observed MW: 160 kDa
This blot was developed with high sensitivity substrate
WB result of ROCK1+ROCK2 Recombinant Rabbit mAb
Primary antibody: ROCK1+ROCK2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 158 kDa
Observed MW: 160 kDa
FC
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling ROCK1+ROCK2 antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) labelling ROCK1+ROCK2 antibody at 1/50 dilution (1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
IP
ROCK1+ROCK2 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating ROCK1+ROCK2 in 0.4 mg Jurkat whole cell lysate.
Western blot was performed on the immunoprecipitate using ROCK1+ROCK2 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: Jurkat whole cell lysate 20 µg (Input)
Lane 2: ROCK1+ROCK2 Rabbit mAb IP in Jurkat whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in Jurkat whole cell lysate
Predicted MW: 158 kDa
Observed MW: 160 kDa
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-ROCK1+ROCK2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti-ROCK1+ROCK2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
