WB result of Phospho-IκBα (Ser32/36) Rabbit mAb
Primary antibody: Phospho-IκBα (Ser32/36) Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with Calyculin A (100ng/ml, 30min) and TNF-α (20ng/ml, 5min) whole cell lysate 20 µg
Predicted MW: 36 kDa
Observed MW: 38 kDa
(This blot was developed with high sensitivity substrate)
Phospho-IκBα (Ser32/36) Recombinant Rabbit mAb (S-751-40)
Phospho-IκBα (Ser32/36) Recombinant Rabbit mAb (S-751-40)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-IκBα (Ser32/36) |
| Synonyms | NF-kappa-B inhibitor alpha, I-kappa-B-alpha (IkB-alpha; IkappaBalpha), Major histocompatibility complex enhancer-binding protein MAD3, NFKBIA, IKBA, MAD3, NFKBI |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P25963 |
| Clone Number | S-751-40 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu |
| Predicted Reactivity | Ms, Pg, Pl |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 |
Background
IκBα (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha) is one member of a family of cellular proteins that function to inhibit the NF-κB transcription factor. IκBα inhibits NF-κB by masking the nuclear localization signals (NLS) of NF-κB proteins and keeping them sequestered in an inactive state in the cytoplasm. In addition, IκBα blocks the ability of NF-κB transcription factors to bind to DNA, which is required for NF-κB's proper functioning.
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Western Blot
