WB result of nNOS Recombinant Rabbit mAb
Primary antibody: nNOS Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse liver lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Negative control: mouse liver lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 161 kDa
Observed MW: 160 kDa
nNOS Recombinant Rabbit mAb (S-1641-70)
nNOS Recombinant Rabbit mAb (S-1641-70)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | nNOS |
| Synonyms | Nitric oxide synthase 1; Constitutive NOS; NC-NOS; NOS type I; Neuronal NOS (N-NOS; nNOS); Nitric oxide synthase, brain (bNOS); Peptidyl-cysteine S-nitrosylase NOS1 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | P29475 |
| Clone Number | S-1641-70 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Ms, Rt |
| Predicted Reactivity | Hu, Sh, Rb |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms, Rt |
| IHC-P | 1:200 | Ms, Rt |
Background
Neuronally-derived Nitric Oxide Synthase (nNOS or NOS1) is a type of nitric oxide synthase enzyme. nNOS is mainly found in neurons and plays a crucial role in the central nervous system, particularly in synaptic signaling and plasticity. It is also present in skeletal muscle, where it regulates muscle contractility and local blood flow. nNOS interacts with a number of proteins, including post-synaptic density protein 95 (PSD-95), which is crucial for its localization at the postsynaptic density near the N-methyl-D-aspartate receptor (NMDAR). This interaction is important for coupling calcium influx through NMDA receptors to NO synthesis. nNOS has been implicated in various neuropathological states, including cognitive and motor deficits associated with early synaptic loss. It also negatively regulates neurogenesis under physiological and pathological conditions.
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Western Blot
WB result of nNOS Recombinant Rabbit mAb
Primary antibody: nNOS Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Lane 2: rat brain lysate 20 µg
Negative control: rat liver lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 161 kDa
Observed MW: 160 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-nNOS antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-nNOS antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse skeletal muscle. Anti-nNOS antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-nNOS antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-nNOS antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat skeletal muscle. Anti-nNOS antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
