WB result of Mouse anti-Human CD90 Antibody
Primary antibody: Mouse anti-Human CD90 Antibody at 1/1000 dilution
Lane 1: K562 whole cell lysate 20 µg
Lane 2: U-2 OS whole cell lysate 20 µg
Lane 3: HuT 78 whole cell lysate 20 µg
Negative control: K562 whole cell lysate
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 18 kDa
Observed MW: 23~37 kDa
Mouse anti-Human CD90 Antibody (S-R538)
Mouse anti-Human CD90 Antibody (S-R538)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD90 |
| Synonyms | Thy-1 membrane glycoprotein; CDw90; Thy-1 antigen, THY1 |
| Location | Cell membrane |
| Accession | P04216 |
| Clone Number | S-R538 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1 |
| Application | WB, IHC-P, FCM |
| Reactivity | Hu |
| Positive Sample | U-2 OS, HuT 78, Jurkat |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:200 | Hu |
| FCM | 1:2000 | Hu |
Background
Thy-1 or CD90 (Cluster of Differentiation 90) is a ~25–37 kDa heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen. Thy-1 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of Thy-1 led to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to some of the initial biochemical description and characterization of a vertebrate GPI anchor and also the first demonstration of tissue specific differential glycosylation.
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Western Blot
FC
Flow cytometric analysis of K562 (Human chronic myelogenous leukemia lymphoblast, Left) / Jurkat (Human T cell leukemia T lymphocyte, Right) labelling Human CD90 antibody at 1/2000 dilution (0.1 μg) / (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
Negative control: K562
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-CD90 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
