WB result of Hexokinase II Rabbit pAb
Primary antibody: Hexokinase II Rabbit pAb at 1/1000 dilution
Lane 1: A549 whole cell lysate 20 µg
Lane 2: HCT 116 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: SH-SY5Y whole cell lysate 20 µg
Lane 5: SK-MEL-28 whole cell lysate 20 µg
Lane 6: U-87 MG whole cell lysate 20 µg
Lane 7: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 102 kDa
Observed MW: 110 kDa
Hexokinase II Rabbit Polyclonal Antibody
Hexokinase II Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Hexokinase II |
| Synonyms | Hexokinase-2; Hexokinase type II (HK II); Hexokinase-B; Muscle form hexokinase; HK2 |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Mitochondrion |
| Accession | P52789 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | A549, HCT 116, HeLa, SH-SY5Y, SK-MEL-28, U-87 MG, Jurkat, NIH/3T3, mouse muscle, mouse testis, rat testis |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:250 | Hu, Ms, Rt |
Background
Hexokinase II (HK II) is an enzyme that plays a crucial role in glucose metabolism. It is a member of the hexokinase family, which consists of four members: HK I, HK II, HK III, and HK IV. HK II specifically phosphorylates a six-carbon sugar, known as hexose, to a hexose phosphate. This enzyme is particularly important as it is the first and rate-limiting step of glycolysis, where it converts glucose to glucose-6-phosphate (G-6P). HK II is predominantly found in insulin-sensitive tissues such as the heart, skeletal muscle, and adipose tissues . It is also upregulated in many types of tumors and is associated with the Warburg effect, which is the enhanced aerobic glycolysis observed in cancer cells. Beyond its fundamental role in glycolysis, HK II is increasingly recognized for its role in survival signaling pathways. It controls cellular growth, prevents the mitochondrial death pathway, and enhances autophagy. The expression of HK II is regulated by the Akt/mTOR pathway, and it is phosphorylated by Akt, which leads to increased mitochondrial binding and protection. HK II also binds and inhibits TORC1 to facilitate autophagy in response to glucose deprivation.
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Western Blot
WB result of Hexokinase II Rabbit pAb
Primary antibody: Hexokinase II Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse muscle lysate 20 µg
Lane 3: mouse testis lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 102 kDa
Observed MW: 110 kDa
WB result of Hexokinase II Rabbit pAb
Primary antibody: Hexokinase II Rabbit pAb at 1/1000 dilution
Lane 1: rat testis lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 102 kDa
Observed MW: 110 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti- Hexokinase II antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti- Hexokinase II antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti- Hexokinase II antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti- Hexokinase II antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti- Hexokinase II antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti- Hexokinase II antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti- Hexokinase II antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
