WB result of GSK3β Recombinant Rabbit mAb
Primary antibody: GSK3β Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 46 kDa
GSK3β Recombinant Rabbit mAb (S-1209-102)
GSK3β Recombinant Rabbit mAb (S-1209-102)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | GSK3β |
| Synonyms | Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B; GSK3B |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus, Cell membrane |
| Accession | P49841 |
| Clone Number | S-1209-102 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, A431, MCF7, NIH/3T3, PC-12 |
| Predicted Reactivity | S. citellus |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:500 | Hu, Ms, Rt |
Background
GSK3β, or glycogen synthase kinase-3 beta, is a serine/threonine protein kinase that plays a crucial role in a variety of cellular processes. It is involved in the molecular pathophysiology of many diseases, including cancer, neurodegenerative disorders, and diabetes. GSK3β is known to regulate mitochondrial activity, which is essential for producing cellular energy and determining cell fates. It has been implicated in the mediation of mitochondrial functions such as biogenesis, bioenergetics, permeability, motility, and apoptosis. In the context of cancer, GSK3β has a dual role. It can act as a tumor suppressor by inhibiting the Wnt/β-catenin signaling pathway, which is often upregulated in various cancers. However, it can also promote tumorigenesis under certain conditions, such as when it phosphorylates and stabilizes β-catenin, leading to uncontrolled cell proliferation. GSK3β is involved in cell survival, proliferation, invasion, and therapy resistance in cancer cells. It also plays a role in the self-renewal and maintenance of cancer stem cells, which are responsible for tumor recurrence and metastasis. GSK3β is also a key player in the regulation of the cell cycle and apoptosis. It phosphorylates various proteins involved in these processes, such as cyclin D1, MYC, BAX, and NF-κB. Inhibition of GSK3β has been shown to induce apoptosis and mitotic catastrophe in cancer cells by disrupting centrosome regulation.
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Western Blot
WB result of GSK3β Recombinant Rabbit mAb
Primary antibody: GSK3β Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 46 kDa
WB result of GSK3β Recombinant Rabbit mAb
Primary antibody: GSK3β Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47 kDa
Observed MW: 46 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-GSK3β antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-GSK3β antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-GSK3β antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-GSK3β antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-GSK3β antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-GSK3β antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
