WB result of Glutamine Synthetase (GS) Rabbit mAb
Primary antibody: Glutamine Synthetase (GS) Rabbit mAb at 1/1000 dilution
Lane 1: SK-MEL-28 whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Negative control: SK-MEL-28 whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 42 kDa
Glutamine Synthetase (GS) Recombinant Rabbit mAb (SDT-549-25)
Glutamine Synthetase (GS) Recombinant Rabbit mAb (SDT-549-25)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Glutamine Synthetase (GS) |
| Synonyms | Glutamate--ammonia ligase, Palmitoyltransferase GLUL, GLUL, GLNS |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cell membrane |
| Accession | P15104 |
| Clone Number | SDT-549-25 |
| Antibody Type | Recombinant mAb |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Fs, Mq, Av, Pg, Hm, Dg, Bv |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | |
| IHC | 1:500 |
Background
Glutamine synthetase (GS) is a 42 kDa protein. GS converts glutamate and ammonia into glutamine using adenosine triphosphate (ATP). GS exerts critical biological functions in liver, kidney, skeletal muscle, and brain. In cancer, GS plays a pivotal role in restructuring the cell metabolism in order to enable continued proliferation and survival of malignant cells in poorly vascularized and nutrient-deprived environments. GS usually combines with Glypican and HSP70 for differentiated diagnosis of liver hypoperia nodules and hepatocellular carcinoma.
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Western Blot
WB result of Glutamine Synthetase (GS) Rabbit mAb
Primary antibody: Glutamine Synthetase (GS) Rabbit mAb at 1/5000 dilution
Lane 1: mouse brain lysate 5 µg
Lane 2: mouse liver lysate 5 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 42 kDa
WB result of Glutamine Synthetase (GS) Rabbit mAb
Primary antibody: Glutamine Synthetase (GS) Rabbit mAb at 1/5000 dilution
Lane 1: rat brain lysate 5 µg
Lane 2: rat liver lysate 5 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 42 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti- Glutamine Synthetase (GS) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-Glutamine Synthetase (GS) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human breast. Anti- Glutamine Synthetase (GS) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Glutamine Synthetase (GS) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human glioma. Anti-Glutamine Synthetase (GS) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Glutamine Synthetase (GS) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Glutamine Synthetase (GS) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Glutamine Synthetase (GS) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-Glutamine Synthetase (GS) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-Glutamine Synthetase (GS) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Glutamine Synthetase (GS) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat liver. Anti-Glutamine Synthetase (GS) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
