Standard curve
Example of Recombinant Trypsin standard curve in Assay Diluent DA2.
Recombinant Trypsin (rTrypsin) OneStep ELISA Kit
Recombinant Trypsin (rTrypsin) OneStep ELISA Kit
Price:
Regular price
$500 USD
Regular price
Sale price
$500 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Antigen | Recombinant Trypsin (rTrypsin) |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Others |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8°C as supplied. |
Kit
| Precision | Intra-assay: 2.7%； Inter-assay: 4.2% |
| Sample type | Cell culture supernatant; Serum; Heparin Plasma, Citrate Plasma, Cell culture extracts, Tissue extracts |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 4.350 pg/mL |
| Range | 7.81 pg/mL – 500 pg/mL |
| Recovery | Cell culture supernatant: 97% (Media is 1640 containing 10% fetal bovine serum) Serum: 108% Heparin Plasma: 92% Citrate Plasma: 86% Cell culture extracts: 105% Tissue extracts: 96% |
| Assay time | 60 minutes |
| Species reactivity | Porcine |
Background
Recombinant Trypsin (rTrypsin) is a genetically engineered serine protease (EC 3.4.21.4) produced via recombinant DNA technology, most commonly in microbial host systems such as Pichia pastoris or E. coli, to yield an enzyme that is identical in amino acid sequence to native porcine or bovine trypsin while ensuring an animal-free production process free from contaminants like chymotrypsin and risks of viral or transmissible spongiform encephalopathy (TSE) agents. As a key biocatalyst, it specifically hydrolyzes peptide bonds at the carboxyl termini of lysine and arginine residues, a specificity determined by its conserved catalytic triad (His57, Asp102, Ser195) and the substrate-binding S1 pocket, with its structural integrity maintained by a calcium-binding loop.
Picture
Picture
ELISA
Linearity
The concentrations of Recombinant Trypsin (rTrypsin) were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: 0.25 % Trypsin-EDTA (2.5 g/L trypsin, 0.38 g/L EDTA·4Na) was 6.25×104 fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 33.52 μg/mL in 0.25 % Trypsin-EDTA.
To assess the linearity of the assay, four samples were spiked with high concentrations of Recombinant Trypsin (rTrypsin) in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Specificity
Test the specificity of different serum.
Leading Competitor comparison
Protocol Diagram
