Standard curve
Example of UltraNuclease standard curve in Assay Diluent A1.
UltraNuclease OneStep ELISA Kit
UltraNuclease OneStep ELISA Kit
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$500 USD
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Product Details
Product Details
Product Specification
| Antigen | UltraNuclease |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Others |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8°C as supplied. |
Kit
| Precision | Intra-assay: 4.1% Inter-assay: 8.5% |
| Sample type | Cell culture supernatant |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 2.424 pg/mL |
| Range | 3.91 pg/mL – 250 pg/mL |
| Recovery | Cell culture supernatant: 96% |
| Assay time | 60 minutes |
| Species reactivity | Others |
Background
UltraNuclease, also called Benzonase, broad-spectrum nucleic acid enzyme, is a non-specific endonuclease derived from Serratia marcescens. It can degrade all forms of RNA and DNA (double-stranded, single-stranded, linear, circular) and is widely used to remove nucleic acids from biological products. Therefore, it is necessary to detect and limit the residual nuclease in biological products to prevent them from affecting.
Picture
Picture
ELISA
To assess the linearity of the assay, two samples were spiked with high concentrations of UltraNuclease in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Protocol Diagram
