WB result of FUS Recombinant Rabbit mAb
Primary antibody: FUS Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Lane 3: SH-SY5Y whole cell lysate 20 µg
Lane 4: THP-1 whole cell lysate 20 µg
Lane 5: Caco-2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 60 kDa
FUS Recombinant Rabbit mAb (S-1015-21)
FUS Recombinant Rabbit mAb (S-1015-21)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | FUS |
| Synonyms | RNA-binding protein FUS; 75 kDa DNA-pairing protein; Oncogene FUS; Oncogene TLS; POMp75; Translocated in liposarcoma protein; TLS |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P35637 |
| Clone Number | S-1015-21 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IP |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HepG2, K562, SH-SY5Y, THP-1, Caco-2, NIH/3T3, C6 |
| Predicted Reactivity | Bv |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IP | 1:50 | Hu |
| IHC-P | 1:250 | Hu, Ms, Rt |
Background
FUS (Fused in Sarcoma) is a multifunctional, predominantly nuclear DNA/RNA binding protein that plays a significant role in various cellular processes, including transcription, RNA splicing, and mRNA transport1. It belongs to the FET (FUS/EWS/TAF15) family of proteins and contains several functional domains that facilitate its interactions with nucleic acids, such as an RNA recognition motif (RRM) and a zinc finger domain. Mutations or abnormal expression of FUS have been implicated in several neurodegenerative diseases, most notably Amyotrophic Lateral Sclerosis (ALS) and Frontotemporal Lobar Degeneration (FTLD). In these diseases, FUS protein tends to form insoluble aggregates in neurons, which is thought to contribute to neuronal dysfunction and degeneration. The exact mechanisms by which FUS contributes to disease pathology are still under investigation, but it is believed that the protein's role in RNA metabolism and its mislocalization to the cytoplasm may be key factors.
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Western Blot
WB result of FUS Recombinant Rabbit mAb
Primary antibody: FUS Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 60 kDa
WB result of FUS Recombinant Rabbit mAb
Primary antibody: FUS Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 60 kDa
IP
FUS Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating FUS in 0.4 mg K562 whole cell lysate.
Western blot was performed on the immunoprecipitate using FUS Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: K562 whole cell lysate 20 µg (Input)
Lane 2: FUS Rabbit mAb IP in K562 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in K562 whole cell lysate
Predicted MW: 53 kDa
Observed MW: 60 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-FUS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-FUS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-FUS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-FUS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-FUS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-FUS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-FUS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-FUS antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
