Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) labelled with FITC Mouse Anti-Human CD19 antibody (Right panel) at 5 μl/test compared with Mouse monoclonal IgG isotype control (Left panel). Then cells were stained with CD3 - Alexa Fluor® 647 separately. Gated on total viable cells.
FITC Mouse Anti-Human CD19 Antibody (S-1039-46)
FITC Mouse Anti-Human CD19 Antibody (S-1039-46)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD19 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P15391 |
| Clone Number | S-1039-46 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu, CyMk |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 0.1% BSA, 0.01% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5 μl per million cells in 100μl volume | Hu, CyMk |
Background
The CD19 antigen plays an important role in clinical oncology. In normal cells, it is the most ubiquitously expressed protein in the B lymphocyte lineage. CD19 expression is induced at the point of B lineage commitment during the differentiation of the hematopoietic stem cell, and its expression continues through preB and mature B cell differentiation until it is finally down-regulated during terminal differentiation into plasma cells. CD19 expression is maintained in B-lineage cells that have undergone neoplastic transformation, and therefore CD19 is useful in diagnosis of leukemias and lymphomas using monoclonal antibodies (mAbs) and flow cytometry.
Picture
Picture
FC
Flow cytometric analysis of CD19 expression on cynomolgus monkey peripheral blood cells. Cynomolgus monkey peripheral blood cells were stained with SDT FITC Mouse Anti- Human CD19 antibody at 5 μl/test (left panel) or FITC Mouse Anti-Human CD19 antibody (right panel). Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
