Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. The EC50 for this effect is less than 120ng/ml.
Fibronectin-CH296 Protein, Human
Fibronectin-CH296 Protein, Human
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$48 USD
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | Fibronectin, FN, CIG, FNZ, MSF |
| Accession | P02751-15 |
| Amino Acid Sequence | Pro1361-Ser1637&Ala1812-Thr2107 |
| Expression System | E.coli |
| Molecular Weight | 58kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE& HPLC |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | 20mM Tris, 200mM NaCl, 5% Trehalose, 0.02% Tween-20, pH8.5 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | Zhou Y. et al. (2020) The effect and clinical significance of FN1 expression on biological functions of gastric cancer cells. Cell Mol Biol (Noisy-le-grand). 66(5): 191-198. |
Background
FN (Fibronectin) is a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. It is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. In recent years, FN has been shown to play an important role in the regulation of various malignant tumors such as lung cancer, colorectal cancer, and ovarian cancer.
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Bioactivity
SDS-PAGE
1μg (R: reducing condition, N: non-reducing condition).
RP-HPLC
>95% as determined by RP-HPLC.