WB result of DFNA5/GSDME Recombinant Rabbit mAb
Primary antibody: DFNA5/GSDME Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: LNCaP whole cell lysate 20 µg
Lane 2: SH-SY5Y whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Weak expression: LNCaP whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 55 kDa
Observed MW: 55 kDa
DFNA5/GSDME Recombinant Rabbit mAb (S-1703-1)
DFNA5/GSDME Recombinant Rabbit mAb (S-1703-1)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | DFNA5/GSDME |
| Synonyms | Gasdermin-E; Inversely correlated with estrogen receptor expression 1 (ICERE-1); Non-syndromic hearing impairment protein 5; ICERE1 |
| Immunogen | Synthetic Peptide |
| Accession | O60443 |
| Clone Number | S-1703-1 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | SH-SY5Y, HeLa |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:250 | Hu |
Background
GSDME (Gasdermin E), also known as DFNA5 or ICERE-1, is a protein found in human cells and is a member of the Gasdermin family. GSDME has membrane pore-forming activity, and its primary mechanism of action involves being activated by Caspase 3 cleavage, which induces a switch from apoptosis to pyroptosis in cells. GSDME is associated with the development of neurodegenerative diseases, and its expression in cochlear hair cells is related to the progression of sensorineural hearing loss. GSDME plays a role in regulating the inflammatory response, and its activation can lead to the release of inflammatory factors, such as IL-1β and IL-18. GSDME is also a potential target in cancer immunotherapy. It is highly expressed in normal tissues but has low expression in certain cancer tissues, which may be related to its role in tumor suppression. The expression level of GSDME can affect the efficacy of chemotherapeutic drugs, and its absence may reduce the effectiveness of some chemotherapy drugs.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-DFNA5/GSDME antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-DFNA5/GSDME antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-DFNA5/GSDME antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
