WB result of DCTN2 Rabbit mAb
Primary antibody: DCTN2 Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: A375 whole cell lysate 20 µg
Lane 3: Saos-2 whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Lane 5: K562 whole cell lysate 20 µg
Lane 6: SH-SY5Y whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 48 kDa
DCTN2 Recombinant Rabbit mAb (S-1082-7)
DCTN2 Recombinant Rabbit mAb (S-1082-7)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Synonyms | Dynactin subunit 2, 50 kDa dynein-associated polypeptide, Dynactin complex 50 kDa subunit (DCTN-50), p50 dynamitin, DCTN50 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cytoskeleton |
| Accession | Q13561 |
| Clone Number | S-1082-7 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC, IP |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Pg, Xe, Bv, Av |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| ICC | 1:500 | |
| IP | 1:50 |
Background
DCTN2, also known as Dynactin 2, is an important protein involved in cellular transport. DCTN2 is a component of the Dynactin complex, which is associated with the microtubule system in cells. It serves as a bridge between the Dynactin complex and Dynein, a protein responsible for transporting cellular organelles, RNA, and proteins along microtubules. The Dynactin complex, which includes DCTN2, is essential for various cellular functions such as the transport of the endoplasmic reticulum to the Golgi apparatus, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. DCTN2 plays a crucial role in the positioning and transport of organelles within the cell. By interacting with other components, it regulates the normal functioning of the cell. DCTN2 is also involved in the formation and maintenance of flagella, which are cellular protrusions responsible for cell movement and environmental sensing.
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Picture
Western Blot
WB result of DCTN2 Rabbit mAb
Primary antibody: DCTN2 Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 48 kDa
WB result of DCTN2 Rabbit mAb
Primary antibody: DCTN2 Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 48 kDa
IP
DCTN2 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating DCTN2 in 0.4 mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using DCTN2 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 20 µg (Input)
Lane 2: DCTN2 Rabbit mAb IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
Predicted MW: 44 kDa
Observed MW: 48 kDa
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-DCTN2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
