WB result of Cyclophilin B Recombinant Rabbit mAb
Primary antibody: Cyclophilin B Recombinant Rabbit mAb at 1/5000 dilution
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: U-87 MG whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 24 kDa
Observed MW: 22 kDa
Cyclophilin B Recombinant Rabbit mAb (S-1543-63)
Cyclophilin B Recombinant Rabbit mAb (S-1543-63)
Price:
Regular price
$130.00 SGD
Regular price
Sale price
$130.00 SGD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Cyclophilin B |
| Synonyms | Peptidyl-prolyl cis-trans isomerase B, PPIase B, CYP-S1, Cyclophilin B, Rotamase B, S-cyclophilin (SCYLP), PPIB, CYPB |
| Immunogen | Synthetic Peptide |
| Location | Endoplasmic reticulum |
| Accession | P23284 |
| Clone Number | S-1543-63 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt, Dg |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | null |
| IHC-P | 1:1000 | null |
Background
Cyclophilin B, also known as CypB or PPIB, is an enzyme that belongs to the family of peptidyl-prolyl isomerases (PPIases). It plays a vital role in protein folding, a process that is essential for the proper functioning of proteins within the cell. Cyclophilin B is involved in various cellular processes, including the immune response, and has been implicated in the pathogenesis of several diseases, such as cancer and viral infections. In the context of viral infections, Cyclophilin B has been shown to interact with the hepatitis C virus (HCV) NS5B polymerase, enhancing its RNA-binding activity, which is crucial for viral replication. Inhibitors of cyclophilin B have demonstrated antiviral activity against HCV, both in vitro and in vivo, suggesting a potential therapeutic target for the treatment of HCV infection. Moreover, Cyclophilin B has been reported to be overexpressed in various cancers, including non-small cell lung carcinoma, hepatocellular carcinoma, and pancreatic carcinoma. Its overexpression has been correlated with reduced patient survival, indicating a possible role in tumor progression.
Picture
Picture
Western Blot
WB result of Cyclophilin B Recombinant Rabbit mAb
Primary antibody: Cyclophilin B Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 24 kDa
Observed MW: 22 kDa
WB result of Cyclophilin B Recombinant Rabbit mAb
Primary antibody: Cyclophilin B Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: rat liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 24 kDa
Observed MW: 22 kDa
WB result of Cyclophilin B Recombinant Rabbit mAb
Primary antibody: Cyclophilin B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: dog liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 24 kDa
Observed MW: 22 kDa
WB result of Cyclophilin B Recombinant Rabbit mAb
Primary antibody: Cyclophilin B Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: chicken liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 24 kDa
Observed MW: 22 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human liver. Anti- Cyclophilin B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti- Cyclophilin B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti- Cyclophilin B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti- Cyclophilin B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti- Cyclophilin B antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
