1μg (R: reducing condition, N: non-reducing condition).
CD48 His Tag Protein, Human
CD48 His Tag Protein, Human
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$500.00 USD
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$500.00 USD
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Product Details
Product Details
Product Specification
| Species | Human |
| Antigen | CD48 |
| Synonyms | BCM1, SLAMF2, BLAST, BLAST1, MEM-102, TCT.1, BCM-1, SLAMF-2 |
| Accession | P09326-1 |
| Amino Acid Sequence | Gln27-Ser220, with C-terminal 8*His Tag QGHLVHMTVVSGSNVTLNISESLPENYKQLTWFYTFDQKIVEWDSRKSKYFESKFKGRVRLDPQSGALYISKVQKEDNSTYIMRVLKKTGNEQEWKIKLQVLDPVPKPVIKIEKIEDMDDNCYLKLSCVIPGESVNYTWYGDKRPFPKELQNSVLETTLMPHNYSRCYTCQVSNSVSSKNGTVCLSPPCTLARSGGGSHHHHHHHH |
| Expression System | HEK293 |
| Molecular Weight | 35-45kDa |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1. Shannon L McArdel. Roles of CD48 in regulating immunity and tolerance. Clin Immunol. 2016 Mar; 164:10-20.Epub 2016 Jan 18. |
Background
CD48, a member of the signaling lymphocyte activation molecule family, participates in adhesion and activation of immune cells. Although constitutively expressed on most hematopoietic cells, CD48 is upregulated on subsets of activated cells. CD48 can have activating roles on T cells, antigen presenting cells and granulocytes, by binding to CD2 or bacterial FimH, and through cell intrinsic effects. Interactions between CD48 and its high affinity ligand CD244 are more complex, with both stimulatory and inhibitory outcomes. CD244:CD48 interactions regulate target cell lysis by NK cells and CTLs, which are important for viral clearance and regulation of effector/memory T cell generation and survival.
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SDS-PAGE
ELISA
Immobilized CD48 His Tag, Human (Cat. No. UA010595) at 2.0μg/mL (100μL/well) can bind 2B4/CD244 Fc Chimera, Human(Cat. No. UA010890) with EC50 of 36.94-47.14 ng/mL.
SPR
Protein A Chip captured 2B4/CD244 Fc Chimera, Human (Cat. No. UA010890), can bind CD48 His Tag, Human(Cat. No. UA010595) with an affinity constant of 17.70nM as determined in SPR assay.
