WB result of CD3ε Recombinant Rabbit mAb
Primary antibody: CD3ε Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Lane 2: EL4.IL2 whole cell lysate 20 µg
Negative control: C2C12 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 21 kDa
CD3ε Recombinant Rabbit mAb (S-300-125)
CD3ε Recombinant Rabbit mAb (S-300-125)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD3ε |
| Synonyms | T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | P22646 |
| Clone Number | S-300-125 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Ms |
| Positive Sample | C2C12, EL4.IL2, mouse spleen |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms |
| IHC-P | 1:1000 | Ms |
Background
CD3ε, also known as the epsilon chain of the CD3 complex, is a crucial component of the T cell receptor (TCR) complex, which plays a central role in T cell activation and antigen recognition. CD3ε is particularly important in the context of T cell-mediated immune responses. It has been identified as a key target in the development of immunotherapies for cancer. Recent research has also highlighted the role of CD3ε in CAR-T cell therapy. By incorporating the CD3ε cytoplasmic domain into a chimeric antigen receptor (CAR), the antitumor activity of CAR-T cells can be improved.
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Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-CD3ε antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse cerebral cortex. Anti-CD3ε antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
