WB result of Caspr Recombinant Rabbit mAb
Primary antibody: Caspr Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: SH-SY5Y whole cell lysate 20 µg
Lane 3: U-87 MG whole cell lysate 20 µg
Lane 4: IMR-32 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 156 kDa
Observed MW: 200 kDa
Caspr Recombinant Rabbit mAb (S-1706-1)
Caspr Recombinant Rabbit mAb (S-1706-1)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Caspr |
| Synonyms | Contactin-associated protein 1; Caspr1; Neurexin IV; Neurexin-4; p190; CNTNAP1; NRXN4 |
| Immunogen | Synthetic Peptide |
| Location | Membrane, Cell junction |
| Accession | P78357 |
| Clone Number | S-1706-1 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, SH-SY5Y, U-87 MG, IMR-32, Neuro-2a, PC-12, rat brain |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20°C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:200 | Hu, Ms, Rt |
| IF | 1:200 | Hu, Ms, Rt |
Background
Caspr, also known as contactin-associated protein (CNTNAP), is a transmembrane protein that plays a crucial role in the nervous system. It is particularly important for the formation and maintenance of the myelin sheath in both the central and peripheral nervous systems. Caspr is expressed at high levels in the paranodal regions of myelinated axons, where it contributes to the formation of septate-like junctions between axons and myelinating cells. Caspr is also essential for the proper localization of voltage-gated potassium channels (VGKC) in the nervous system. It helps in the aggregation and development of K channels (Kv1.1 and Kv1.2) in the proximal nodal region, which is crucial for axon formation and stability. Furthermore, Caspr is a component of the paranodal junctions, which are critical for maintaining the integrity of myelinated axons. It helps in the reciprocal signaling between axons and glial cells, ensuring proper axonal function.
Picture
Picture
Western Blot
WB result of Caspr Recombinant Rabbit mAb
Primary antibody: Caspr Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 156 kDa
Observed MW: 200 kDa
WB result of Caspr Recombinant Rabbit mAb
Primary antibody: Caspr Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 156 kDa
Observed MW: 200 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Caspr antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Caspr antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Caspr antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded human cerebral cortex. Anti-Caspr antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse cerebral cortex. Anti- Caspr antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat cerebral cortex. Anti- Caspr antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
