• ADORA2A (wild type) His-Flag-Twinstrep Tag Protein,  Human (Detergent)
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ADORA2A (wild type) His-Flag-Twinstrep Tag Protein,  Human (Detergent)

ADORA2A (wild type) His-Flag-Twinstrep Tag Protein,  Human (Detergent)

Catalog Number: UA060014 Reactivity: Human Conjugation: Unconjugated Brand: UA BIOSCIENCE
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$1,144 USD
$1,144 USD
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Product Details

Product Specification


Species Human
Synonyms ADORA2A, adenosine A2a receptor, A2aR, ADORA2, RDC8
Accession P29274
Concentration 1mg/ml by Nanodrop
Amino Acid Sequence

Full length (wt), and Flag tag at N-Terminal, and His-Twinstrep tag at C-Terminal
Expression System CHO
Molecular Weight 52.7 kDa
Purity ＞95% by SDS-PAGE
Endotoxin <0.1EU/μg
Conjugation Unconjugated
Tag His Tag, Strep Tag
Physical Appearance Liquid
Storage Buffer

20 mM Hepes, pH 8.0, 100 mM NaCl, 0.05 % DDM, 0.01 % CHS
Stability & Storage

·6 months from date of receipt, -60 to -80 °C as supplied.
·about 3 days in 2 to 8 °C.
·Please avoid repeated freeze-thaw cycles.
Reference

1. Lebon G, Warne T, Edwards P C, et al. Agonist-bound adenosine A2A receptor structures reveal common features of GPCR activation[J]. Nature, 2011, 474(7352): 521-525.

Background

The Adenosine Receptor A2AR (ADORA2A) is a member of the G protein-coupled receptor (GPCR) superfamily. GPCRs are seven-transmembrane proteins that transduce extracellular signals into intracellular responses. A2AR binds adenosine as its endogenous agonist and interacts with Gs and Golf proteins to increase intracellular cAMP levels. This receptor plays crucial roles in cardiac rhythm, cerebral and renal blood flow, immune function, pain modulation, and sleep regulation. It is also associated with pathophysiological conditions such as inflammatory diseases and neurodegenerative disorders. The A2AR receptor is a key player in multiple physiological processes and holds significant potential as a therapeutic target. The A2AR protein product may enhance drug discovery efforts.

Picture

SDS-PAGE

1μg (R: reducing condition, N: non-reducing condition).

ELISA

Immobilized ADORA2A (wild type) His-Flag-Twinstrep tag Protein, Human (Detergent) (Cat. No. UA060014) at 2 μg/mL (100 μL/well) on a Nickel Coated plate can bind Mouse Anti-ADORA2A with EC50 of 7.16-11.40ng/mL.