The PDE4D7 activity was quantified using a FP-based assay. The reaction was performed by incubating the PDE4D7 protein and FAM-Cyclic-3,5-AMP at 25℃ for 60 min, then adding the binding reagent, finally measuring FP signal on BMG.
Product Details
Product Details
Product Specification
Species | Human |
Synonyms | 3',5'-cyclic-AMP phosphodiesterase 4D,DPDE3 |
Accession | Q08499-11 |
Amino Acid Sequence | K2-T748(end); N-terminal GST-tag-3C |
Expression System | Baculovirus-InsectCells |
Molecular Weight | 111.4 kDa |
Purity | >80% by SDS-PAGE |
Conjugation | Unconjugated |
Tag | GST Tag |
Physical Appearance | Liquid |
Storage Buffer | 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5 |
Stability & Storage | Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles. |
Picture
Picture
Bioactivity
SDS-PAGE
