The PDE4D7 activity was quantified using a FP-based assay. The reaction was performed by incubating the PDE4D7 protein and FAM-Cyclic-3,5-AMP at 25℃ for 60 min, then adding the binding reagent, finally measuring FP signal on BMG.
Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | 3',5'-cyclic-AMP phosphodiesterase 4D,DPDE3 |
| Accession | Q08499-11 |
| Amino Acid Sequence | K2-T748(end); N-terminal GST-tag-3C |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | 111.4 kDa |
| Purity | >80% by SDS-PAGE |
| Conjugation | Unconjugated |
| Tag | GST Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5 |
| Stability & Storage | Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles. |
Picture
Picture
Bioactivity
SDS-PAGE
