2μg（R: reducing conditions）
Mycobacterium tuberculosis fbpA/Ag85A Protein, His Tag
Mycobacterium tuberculosis fbpA/Ag85A Protein, His Tag
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Product Details
Product Details
Product Specification
| Synonyms | Diacylglycerol acyltransferase/mycolyltransferase Ag85A, DGAT, Acyl-CoA:diacylglycerol acyltransferase, Antigen 85 complex A (85A; Ag85A), Fibronectin-binding protein A (Fbps A), mpt44, MT3911 |
| Accession | P9WQP2 |
| Amino Acid Sequence | Protein sequence (P9WQP2, Phe44-Ala338, with N-His Tag) FSRPGLPVEYLQVPSPSMGRDIKVQFQSGGANSPALYLLDGLRAQDDFSGWDINTPAFEWYDQSGLSVVMPVGGQSSFYSDWYQPACGKAGCQTYKWETFLTSELPGWLQANRHVKPTGSAVVGLSMAASSALTLAIYHPQQFVYAGAMSGLLDPSQAMGPTLIGLAMGDAGGYKASDMWGPKEDPAWQRNDPLLNVGKLIANNTRVWVYCGNGKPSDLGGNNLPAKFLEGFVRTSNIKFQDAYNAGGGHNGVFDFPDSGTHSWEYWGAQLNAMKPDLQRALGATPNTGPAPQGA |
| Expression System | HEK293 |
| Molecular Weight | Predicted MW: 33.3 kDa Observed MW: 33 kDa |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4 with 3% trehalose. |
| Reconstitution | Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation. |
| Stability & Storage | 12 months from date of receipt, -20 to -70 °C as supplied. |
Background
Ag85A, a member of the Antigen 85 complex, is a pivotal mycolyltransferase secreted by Mycobacterium tuberculosis and other mycobacteria. This enzyme is essential for constructing the unique, impermeable mycobacterial cell wall. It catalyzes the transfer of mycolic acids—long-chain fatty acids critical for pathogenicity—onto the arabinogalactan layer of the cell envelope, forming a protective lipid barrier. Ag85A also exhibits diacylglycerol acyltransferase activity, contributing to trehalose dimycolate (cord factor) synthesis. As one of the most abundant and immunodominant proteins secreted by the bacillus, Ag85A is a major target of the host immune response and a leading candidate for subunit vaccines against tuberculosis.
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