WB result of TOX Recombinant Rabbit mAb
Primary antibody: TOX Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A549 whole cell lysate 20 µg
Lane 2: Raji whole cell lysate 20 µg
Low expression control: A549 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 58 kDa
Observed MW: 60-70 kDa
This blot was developed with high sensitivity substrate
TOX Recombinant Rabbit mAb (S-2296-44)
TOX Recombinant Rabbit mAb (S-2296-44)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | TOX |
| Synonyms | Thymocyte selection-associated high mobility group box protein TOX; Thymus high mobility group box protein TOX; KIAA0808 |
| Immunogen | Recombinant Protein |
| Location | Nucleus |
| Accession | O94900 |
| Clone Number | S-2296-44 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | Raji, mouse spleen, mouse thymus, rat spleen, rat thymus |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500-1:1000 | Hu |
| IHC-P | 1:250 | Hu |
Background
TOX protein is a transcription factor that plays a crucial role in the immune system. It is involved in the regulation of T cell differentiation and function, particularly in the development and maintenance of regulatory T cells (Tregs) which are essential for maintaining immune tolerance and preventing autoimmunity. Additionally, TOX has been implicated in the response of T cells to chronic antigen stimulation, such as in the context of chronic infections or cancer, where it may contribute to T cell exhaustion. Its expression and activity can influence the balance between effector and regulatory T cell populations, thereby having a significant impact on the overall immune response.
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Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human thymus. Anti-TOX antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-TOX antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-TOX antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse thymus. Anti-TOX antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-TOX antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-TOX antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
